fibroblasts  (Cell Applications Inc)

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effects of different Dicliptera chinensis polysaccharide (DCP) concentrations on the viability of rat dermal fibroblasts (RDFs). CCK8 assay was used to examine the role of DCP in RDF cell lines. Cells were inoculated into 96-well plates and exposed to the specified concentrations of DCP (50 μg/mL, 100 μg/mL, 200 μg/mL, 400 μg/mL, 800 μg/mL, and 1.6 mg/mL) for 24 hours. The result shown is the standard deviation average of 3 independent experiments. The significance was analyzed using one-way analysis of variance with Dunnett-t test to compare the means between the control and experimental groups (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and blank group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: International Dental Journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Effect of Dicliptera chinensis polysaccharide (DCP) on radiation-induced apoptosis of rat dermal fibroblasts (RDFs). (A) RDFs were cultured in 100 μg/mL and 200 μg/mL DCP with experimental concentrations for 24 hours and then irradiated with 8 GY X-rays. The cells were then stained with Annexin V- Fluorescein Isothiocyanate and propium iodide and detected by flow cytometry. (B) The result shown in B is the standard deviation average of 3 independent experiments. The significance was determined using one-way analysis of variance (* P < .05, ⁎⁎ P < .01, ⁎⁎⁎ P < .001 and ionising radiation [IR] group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: Cell Culture, Irradiation, Staining, Flow Cytometry, Standard Deviation

Journal: International dental journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway.

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Figure 1 – Effects of different Dicliptera chinensis polysaccharide (DCP) concentrations on the viability of rat dermal fibro- blasts (RDFs). CCK8 assay was used to examine the role of DCP in RDF cell lines. Cells were inoculated into 96-well plates and exposed to the specified concentrations of DCP (50 mg/mL, 100 mg/mL, 200 mg/mL, 400 mg/mL, 800 mg/mL, and 1.6 mg/mL) for 24 hours. The result shown is the standard deviation average of 3 independent experiments. The significance was analyzed using one-way analysis of variance with Dunnett-t test to compare the means between the control and experimental groups (*P < .05, **P < .01, *** P < .001 and blank group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: CCK-8 Assay, Standard Deviation, Control

Journal: International dental journal

Article Title: Anti-Radiofibrosis Effect of Dicliptera chinensis Polysaccharide on Rat Dermal Fibroblasts Via The TGF-β1/Smads/CTGF Signaling Pathway.

doi: 10.1016/j.identj.2024.09.024

Figure Lengend Snippet: Figure 2 – Effect of Dicliptera chinensis polysaccharide (DCP) on radiation-induced apoptosis of rat dermal fibroblasts (RDFs). (A) RDFs were cultured in 100 mg/mL and 200 mg/mL DCP with experimental concentrations for 24 hours and then irradiated with 8 GY X-rays. The cells were then stained with Annexin V- Fluorescein Isothiocyanate and propium iodide and detected by flow cytometry. (B) The result shown in B is the standard deviation average of 3 independent experiments. The signifi- cance was determined using one-way analysis of variance (*P < .05, **P < .01, *** P < .001 and ionising radiation [IR] group).

Article Snippet: Primary rat dermal fibroblasts (RDFs) were purchased from Procell Corp.

Techniques: Cell Culture, Irradiation, Staining, Cytometry, Standard Deviation

Journal: ACS Applied Materials & Interfaces

Article Title: Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development of In Vivo Tissue-Engineered Blood Vessels

doi: 10.1021/acsami.2c05988

Figure Lengend Snippet: General overview of the cellular composition of the tissue capsule formed around implanted rods. (A) vWF staining for vascularization, (B) Ki67 for proliferating cells, (C) α-SMA for myofibroblast, (D) desmin for contractile smooth muscle cells, and (E) vimentin for fibroblast. The tissue capsules are mainly composed of fibroblasts and myofibroblasts. There are barely contractile smooth muscle cells present. All tissue capsules are well vascularized. Scale bar represents 50 μm.

Article Snippet: Adult rat dermal fibroblasts (aRDF, #R2320, ScienCell Research Laboratories) were cultured with a basic culture medium comprising DMEM (Gibco), fetal bovine serum (10%, Lonza), l -glutamine (2 mM, Gibco), and penicillin (100 U/mL) and streptomycin (100 mg/mL, Gibco). aRDF were expanded at an initial seeding density of 5000 cells/cm 2 in a culture medium and refreshed every 2–3 days.

Techniques: Staining, Capsules

Journal: ACS Applied Materials & Interfaces

Article Title: Long-Term Controlled Growth Factor Release Using Layer-by-Layer Assembly for the Development of In Vivo Tissue-Engineered Blood Vessels

doi: 10.1021/acsami.2c05988

Figure Lengend Snippet: (A) The number of cells (per 1000 μm 2 ) positively stained for α-smooth muscle actin is depicted, a differentiation marker for myofibroblasts. (B) The number of cells (per 1000 μm 2 ) stained positively with the desmin antibody is depicted, which stains the intermediate filament protein expressed in contractile smooth muscle cells. (C) The number of cells (per 1000 μm 2 ) positively stained for vimentin is depicted, which is an intermediate filament expressed in fibroblasts. The expressions of myofibroblasts, contractile smooth muscle cells, and fibroblasts were observed in the following conditions: control (no release), burst release (TGF-β1 and PDGF-BB), single layer-by-layer release (TGF-β1, PDGF-BB, and IGF-1), and dual layer-by-layer release (TGF-β1/IGF-1 and PDGF-BB/TGF-β1). Data was analyzed using ordinary one-way ANOVA followed by a post hoc analysis using Tukey’s test, and * indicates significance of P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, and **** P ≤ 0.0001.

Article Snippet: Adult rat dermal fibroblasts (aRDF, #R2320, ScienCell Research Laboratories) were cultured with a basic culture medium comprising DMEM (Gibco), fetal bovine serum (10%, Lonza), l -glutamine (2 mM, Gibco), and penicillin (100 U/mL) and streptomycin (100 mg/mL, Gibco). aRDF were expanded at an initial seeding density of 5000 cells/cm 2 in a culture medium and refreshed every 2–3 days.

Techniques: Staining, Marker, Control